RESEARCH ARTICLE


Pyridoxine Decreases Oxidative Stress on Human Erythrocyte Membrane Protein in vitro



Margarita Velásquez1, Darío Méndez2, Carlos Moneriz1, *
1 Biochemistry and Diseases Research Group, University of Cartagena, Cartagena, Colombia
2 Analytical Chemical and Biomedicine Research Group, University of Cartagena, Cartagena, Colombia


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© 2019 Velásquez et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Biochemistry and Diseases Research Group, University of Cartagena, Cartagena, Colombia;
Tel: 0556698176; Fax: 0556698177; E-mail: cmonerizp@unicartagena.edu.co


Abstract

Background:

Pyridoxine has reduction and prevention against the levels of reactive oxygen species in in vitro studies. However, the biochemical mechanism that explains this behavior has not yet been fully clarified.

Objective:

To evaluate the effect of pyridoxine against oxidative damage on the membrane of human erythrocytes.

Methods:

Cumene hydroperoxide was used to induce oxidative stress in protein and lipid. Human erythrocytes were incubated with pyridoxine and cumene hydroperoxide, either alone or together for 8 h. Oxidative damage was determined by measuring lipid peroxidation and membrane protein carbonylation.

Results:

The results indicate that the malondialdehyde concentration decreased with increasing concentration of pyridoxine. The membrane protein content also decreased with increasing concentration of vitamin B6, which was confirmed by the decreased signal intensity in the western blot when compared to control without pyridoxine. Results demonstrate that pyridoxine can significantly decrease lipid peroxidation and protein carbonylation in red cell membrane exposed to high concentrations of oxidant agent.

Conclusion:

Pyridoxine showed a protective effect against the oxidative stress in human erythrocytes in vitro, inhibiting the carbonylation and the oxidative damage of erythrocyte membrane proteins. To date, such an effect has not yet been reported in terms of protein oxidation.

Keywords: Pyridoxine, B6, Oxidative stress, Antioxidant, Membrane protein, Carbonylation.