Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust

The Open Biochemistry Journal 30 Apr 2019 RESEARCH ARTICLE DOI: 10.2174/1874091X01913010032


Background and Objectives:

Occupational exposure to silica dust can lead to biochemical damage. Malondialdehyde (MDA) can be considered as a primary marker for measuring the level of oxidative stress in a living organism. This study was conducted in order to evaluate the level of MDA in blood and urine of mice exposed to silica dust.

Material and Methods:

In this experimental study, 72 mice (BALB/c) were randomly allocated to five exposed groups and 1 control group. Exposure of mice to pure 99% silica dust was done in closed containers. Blood sampling was performed from the heart of mice and urine sampling fulfilled by insertion into a metabolic cage. The RAO et al. method was used to measure MDA.


The highest level of plasma MDA in group 1 in the 4th month was 8.4±0.41 nmol/l and the lowest level of MDA was 1.3±0.2 nmol/l in the third sampling in the control group, also the highest amount of urine MDA in the first and second groups and 4 months after exposure was 1.16±0.51 nmol/l, and the lowest in the control group and in the third sampling was 0.48±0.06 nmol/l. A significant difference was found between the levels of MDA in all exposed groups at different times except for the 5th group with the lowest concentration (P < 0.05).


MDA in blood and urine could be proposed as a good biomarker for the evaluation of biochemical damages caused by silica dust. Measuring MDA is also a simple and inexpensive method that does not require complex equipment and can be used as an early detection test for biochemical damages caused by silica.

Keywords: Biomarker, Crystalline silica, Dust, Mouse, Malondialdehyde, Biochemical damages.
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