RESEARCH ARTICLE


Altering Amino Acid Profile in Catharanthus Roseus (L.) G. Don Using Potassium and Ascorbic Acid Treatments



Neda Sahi1, Akbar Mostajeran1 , *, Mustafa Ghanadian2
1 Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
2 Institute of Pharmacy, Isfahan University of Medical Science, Isfahan, Iran


© 2021 Sahi et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran; Tel: 00987932471; Fax: 00987932456;
E-mail: mostajerana@yahoo.com


Abstract

Background:

Catharanthus roseus (L.) G. Don is the main source of alkaloids anticancer drugs. Alkaloids are derived from amino acids and can lead to changes in these valuable compounds.

Objective:

This experiment evaluated the variation of amino acids under potassium and ascorbic acid treatments.

Methods:

Different concentrations (1.5, 3.16, 15 and 30 mM) and forms (K2SO4 and KNO3) of potassium (K+) were added to plants via Hoagland’s nutrient solution. Ascorbic acid (AsA) (750 mg L-1) was sprayed on the leaves surfaces on days 68 and 78. Amino acids were extracted from 90-day-old plant leaves, and different amino acids were determined by High-Performance Liquid Chromatography with fluorescence detection.

Results:

Amino acids increased in K+ deficiency (1.5 mM), but changes in negatively charged amino acids were lower. In contrast, N-rich amino acids showed the biggest change. In excessive K+, branched-chain and aromatic amino acids decreased, while the least amount of the other amino acids was observed in the plants treated with optimum K+.

Conclusion:

The exterior of AsA and excessive K+ cause branched-chain and aromatic amino acids, aspartic acid and glutamic acid to decrease.

Keywords: Aromatic amino acids, Branched-chain amino acids, Free amino acid profile, HPLC-fluorescence, Aspartic acid, Glutamic acid.