Altering Amino Acid Profile in Catharanthus Roseus (L.) G. Don Using Potassium and Ascorbic Acid Treatments
Neda Sahi1, Akbar Mostajeran1 , *, Mustafa Ghanadian2
Identifiers and Pagination:Year: 2021
First Page: 53
Last Page: 60
Publisher Id: TOBIOCJ-15-53
Article History:Received Date: 11/5/2021
Revision Received Date: 03/8/2021
Acceptance Date: 09/8/2021
Electronic publication date: 28/10/2021
Collection year: 2021
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Catharanthus roseus (L.) G. Don is the main source of alkaloids anticancer drugs. Alkaloids are derived from amino acids and can lead to changes in these valuable compounds.
This experiment evaluated the variation of amino acids under potassium and ascorbic acid treatments.
Different concentrations (1.5, 3.16, 15 and 30 mM) and forms (K2SO4 and KNO3) of potassium (K+) were added to plants via Hoagland’s nutrient solution. Ascorbic acid (AsA) (750 mg L-1) was sprayed on the leaves surfaces on days 68 and 78. Amino acids were extracted from 90-day-old plant leaves, and different amino acids were determined by High-Performance Liquid Chromatography with fluorescence detection.
Amino acids increased in K+ deficiency (1.5 mM), but changes in negatively charged amino acids were lower. In contrast, N-rich amino acids showed the biggest change. In excessive K+, branched-chain and aromatic amino acids decreased, while the least amount of the other amino acids was observed in the plants treated with optimum K+.
The exterior of AsA and excessive K+ cause branched-chain and aromatic amino acids, aspartic acid and glutamic acid to decrease.