RESEARCH ARTICLE
Altering Amino Acid Profile in Catharanthus Roseus (L.) G. Don Using Potassium and Ascorbic Acid Treatments
Neda Sahi1, Akbar Mostajeran1 , *, Mustafa Ghanadian2
Article Information
Identifiers and Pagination:
Year: 2021Volume: 15
First Page: 53
Last Page: 60
Publisher ID: TOBIOCJ-15-53
DOI: 10.2174/1874091X02115010053
Article History:
Received Date: 11/5/2021Revision Received Date: 03/8/2021
Acceptance Date: 09/8/2021
Electronic publication date: 28/10/2021
Collection year: 2021
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
Catharanthus roseus (L.) G. Don is the main source of alkaloids anticancer drugs. Alkaloids are derived from amino acids and can lead to changes in these valuable compounds.
Objective:
This experiment evaluated the variation of amino acids under potassium and ascorbic acid treatments.
Methods:
Different concentrations (1.5, 3.16, 15 and 30 mM) and forms (K2SO4 and KNO3) of potassium (K+) were added to plants via Hoagland’s nutrient solution. Ascorbic acid (AsA) (750 mg L-1) was sprayed on the leaves surfaces on days 68 and 78. Amino acids were extracted from 90-day-old plant leaves, and different amino acids were determined by High-Performance Liquid Chromatography with fluorescence detection.
Results:
Amino acids increased in K+ deficiency (1.5 mM), but changes in negatively charged amino acids were lower. In contrast, N-rich amino acids showed the biggest change. In excessive K+, branched-chain and aromatic amino acids decreased, while the least amount of the other amino acids was observed in the plants treated with optimum K+.
Conclusion:
The exterior of AsA and excessive K+ cause branched-chain and aromatic amino acids, aspartic acid and glutamic acid to decrease.
