Phosphorylation of Tip60 Tyrosine 327 by Abl Kinase Inhibits HAT Activity through Association with FE65



Sung Hwa Shin1, 3, Sang Sun Kang1, 2, *
1 Department of Biology Education, Chungbuk National University, 410 Seongbong Road, Heungdok-gu, Cheongju, Chungbuk, 361-763, Republic of Korea
2 Biotechnology Research Institute, Chungbuk National University, 410 Seongbong Road, Heungdok-gu, Cheongju, Chungbuk, 361-763, Republic of Korea
3 Bio Center, Chungbuk Technopark, Ochang-eup, Cheongwon, Chungbuk, 363-883, Republic of Korea


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© Shin and Kang; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Biology Education, Chungbuk National University, 410 Seongbong Road, Heungdok-gu, Cheongju, Chungbuk, 361-763, Republic of Korea; Tel: +82 43 261 3278; Fax: +82 43 271 0526; E-mail: jin95324@chungbuk.ac.kr


Abstract

The transfer of acetyl groups from acetyl coenzyme A to the ε amino group of internal lysine residues is catalyzed by Tip60, which is in the MYST family of nuclear histone acetyltransferases (HATs). The tyrosine phosphorylation of Tip60 seems to be a unique modification. We present evidence that Tip60 is modified on tyrosine 327 by Abl kinase. We show that this causes functional changes in HAT activity and the subcellular localization of TIP60, which forms a complex with Abl kinase. The Tip60 mutation Y327F abolished tyrosine phosphorylation, reduced the inhibition of Tip60 HAT activity, and caused G0-G1 arrest and association with FE65. Thus, our findings for the first time suggested a novel regulation mechanism of Tip60. Regulation was through phosphorylation of tyrosine 327 by Abl tyrosine kinase and depended on environmental conditions, suggesting that the tyrosine residue of Tip60 is important for the activation process.

Keywords: : TIP60, Fe65, Abl kinase, HAT, phosphorylation.