The Impact of the Level of the Intestinal Short Chain Fatty Acids in Inflammatory Bowel Disease Patients Versus Healthy Subjects

N. Huda-Faujan1, 3, A.S. Abdulamir2, 3, A.B. Fatimah*, 4, O. Muhammad Anas4, M. Shuhaimi5, A.M. Yazid6, Y.Y. Loong7
1 Food Biotechnology Programme, Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
2 Institute of Bioscience, Department of Microbiology
3 Medical Microbiology Department, Faculty of Medicine, Alnahrain University, Iraq
4 Food Science Department, Faculty of Food Science and Technology
5 Halal Products Research Institute
6 Food Service and Management Department, Faculty of Food Science and Technology
7 Medicine Department, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

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© Huda-Faujan et al.; Licensee Bentham Open.

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* Address correspondence to this author at the Food Science Department, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Tel: 00-60-(0)3-89468375; Fax: 00-60-(0)3-89423552; E-mail:


The aim of this study was to determine the changes of short chain fatty acids (SCFAs) in faeces of inflammatory bowel disease (IBD) patients compared to healthy subjects. SCFAs such as pyruvic, lactic, formic, acetic, propionic, isobutyric and butyric acids were analyzed by using high performance liquid chromatography (HPLC). This study showed that the level of acetic, 162.0 µmol/g wet faeces, butyric, 86.9 µmol/g wet faeces, and propionic acids, 65.6 µmol/g wet faeces, decreased remarkably in IBD faecal samples when compared with that of healthy individuals, 209.7, 176.0, and 93.3 µmol/g wet faeces respectively. On the contrary, lactic and pyruvic acids showed higher levels in faecal samples of IBD than in healthy subjects. In the context of butyric acid level, this study also found that the molar ratio of butyric acid was higher than propionic acid in both faecal samples. This might be due to the high intake of starch from rice among Malaysian population. It was concluded that the level of SCFAs differ remarkably between faecal samples in healthy subjects and that in IBD patients providing evidence that SCFAs more likely play an important role in the pathogenesis of IBD.

Keywords: Organic acid, human faecal, IBD, HPLC.