Characterization of the Cdc6 Homologues from the Euryarchaeon

Haugland , Gyri Teien; Innselset, Marte; Madern, Dominique; Birkeland, Nils-Kåre

Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum

Gyri Teien Haugland ^{*, 1}, Marte Innselset², Dominique Madern³, Nils-Kåre Birkeland¹

¹ Department of Biology, University of Bergen, P.O. Box 7800, N-5020 Bergen, Norway

² Department of Biomedicine, University of Bergen, Jonas Lies Vei 91, N-5009 Bergen, Norway

³ Institute of Structural Biology, CEA-CNRS-UJF, 41 Rue Jules Horowitz, 38027 Grenoble, France

Article Information

Identifiers and Pagination:

Year: 2008
Volume: 2
First Page: 129
Last Page: 134
Publisher ID: TOBIOCJ-2-129
DOI: 10.2174/1874091X00802010129

Article History:

Received Date: 10/8/2008
Revision Received Date: 20/8/2008
Acceptance Date: 3/9/2008
Electronic publication date: 27/9/2008
Collection year: 2008

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© Haugland et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

^* Address correspondence to this author at the Department of Biology, Uni-versity of Bergen, P.O. Box 7800, N-5020 Bergen, Norway; Tel: +47 55584882; Fax: +47 55589671; E-mail: Gyri.Haugland@bio.uib.no

Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM

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RESEARCH ARTICLE

Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum

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