RESEARCH ARTICLE
Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum
Gyri Teien Haugland *, 1, Marte Innselset2, Dominique Madern3, Nils-Kåre Birkeland1
Article Information
Identifiers and Pagination:
Year: 2008Volume: 2
First Page: 129
Last Page: 134
Publisher ID: TOBIOCJ-2-129
DOI: 10.2174/1874091X00802010129
Article History:
Received Date: 10/8/2008Revision Received Date: 20/8/2008
Acceptance Date: 3/9/2008
Electronic publication date: 27/9/2008
Collection year: 2008

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http: //creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Abstract
Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM