Biochemical and In-silico Studies on Pectin Methylesterase from G9 Variety of Musa acuminata for Delayed Ripening



Charu Verma 1, 2, Singh R.K3, Ram B Singh 4, Sanjay Mishra *, 1
1 School of Biotechnology, IFTM University, Delhi Road (NH 24), Moradabad 244 102, Uttar Pradesh, India;
2 Department of Biotechnology & Microbiology, Adhunik Institute of Education and Research, 9th Milestone, Duhai , Ghaziabad, UP, India;
3 Department of Electronics, Uttarakhand Technical University, Dehradun, U.K., India;
4 Halberg Hospital & Research Institute, Moradabad 244 001, Uttar Pradesh, India


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© Verma et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the School of Biotechnology, IFTM University, Delhi Road (NH 24), Delhi Road, Moradabad 244 102, Uttar Pradesh, India; E-mail: sanjaymishra@iftmuniversity.ac.in


Abstract

Ripening of fruit is a very important process but in some fruits early ripening leads to a great damage during long distance transportation. There are various biochemical changes taking place during the phase of ripening of fruit such as changes in respiration, aroma, flavor, ethylene production and activity of cell wall degrading enzymes. Some important cell wall degrading enzymes are Polygalacturonase (PG), Pectin methylesterase (PME), Pectin lyase, RGase. PME is known to act as a cell wall hydrolyzing enzyme, responsible for demethyl esterification of cell wall polygalacturonan. The present study includes the biochemical and molecular characterization of PME from Grand naine variety of Musa acuminata (banana). This study also deals with the in-silico study reflecting inhibition of PME activity in context to delayed ripening in banana. It mainly deals with the identification of a PME1 gene from Grand naine variety of banana. The expression of this gene is related with the process of ripening. The expression of PME1 gene was observed to be peaked on 3rd day in ethylene treated samples of banana but the activity in untreated samples called control was rather slow and then there was a sudden decrease in their activity in both treated as well as untreated samples. With the help of in-silico study, we observed that banana has maximum homology with carrot by using cross species analysis.The designed model has been reported to be of good quality on the basis of its verification and validation. The designed model was observed to be appropriate for docking. The information of binding sites of ligand provides new insights into the predictable functioning of relevant protein.

Keywords: Active site, grand naine, fruit ripening, inhibitors, molecular docking, pectin methylesterase.