Does Phenoloxidase Contributed to the Resistance? Selection with Butane-Fipronil Enhanced its Activities from Diamondback Moths

Shouzhu Liu1, 2, Hongtao Niu2, Ting Xiao3, Chaobin Xue2, Zhongde Liu4, Wanchun Luo*, 2
1 College of Agriculture, Liaocheng University, Liaocheng 252059, China
2 College of Plant Protection, Shandong Key Laboratory of Pesticide Toxicology and Application Technique, Shandong Agricultural University, Tai’an 271018, China
3 Institute of Zhenjiang Agricultural Sciences Jiangsu Hill Region, Jurong Jiangsu 212400, China
4 College of life science and technique, Taishan University, Tai’an 271000, China

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© Liu et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the College of Plant Protection, Shandong Agricultural University, Shandong, Tai’an 271018, China; Tel: +86 538 8242983; Fax: +86 538 8242983; E-mail:


Using microtitration method, the relationship between Phenoloxidase activity and the resistance of the diamondback moth Plutella xylostella (Linnaeus) to the novel insecticide butane-fipronil was determined in vitro. After selection of the tenth-generation by butane-fipronil, the resistance of the fourth instar larvae was increased 83.80-fold as compared to the susceptible strain. Phenoloxidase activity of the resistant strain (POr) was 1.29-fold higher than the susceptible one (POs). However, the Km and optimum pH values were similar in resistant and susceptible strains, which were 1.11 mM and 6.5, respectively. Both POr and POs have maximum stability at pH values less than 7.0, although POs was less stable at lower pH values than POr. In addition, the thermal stabilities of the two phenoloxidase were very similar. It is suggested that PO may play an important role in the increasing resistance of pests to pesticides.

Keywords: Butane-fipronil, phenoloxidase, Plutella xylostell, resistance.