RESEARCH ARTICLE


Sphingosine Increases ATP Release From Red Blood Cells



Francesco Misiti1, *
1 Department of Human, Social and Health, University of Cassino and Lazio Meridionale, V. S. Angelo, Loc. Folcara, 03043, Cassino (FR), Italy


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Creative Commons License
© 2022 Francesco Misiti

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Human Sciences, Society and Health, The University of Cassino and Southern Lazio, Italy, V. S.Angelo-Polo didattico della Folcara, Cassino (FR) 03043;
Tel: +39 7762994423; E-mail: f.misiti@unicas.it


Abstract

Background:

RBC plays a pivotal role in oxygen delivery, improving distribution where it needs. When RBC enters a low oxygen area, a mechanism mediated by a signaling pathway releases ATP, responsible for vasodilatation.

Objective:

Clarify the potential role of sphingosine on the release of ATP from RBC.

Methods:

ATP release increases after sphingosine exposure in RBC under low oxygen conditions. ATP release in deoxygenated RBC shows data like that of control RBC: (1) RBC after band 3 modification by 4,4'- diisothio-cyanato-stilbene- 2,2'-disulphonic acid (DIDS); (2) CO-treated RBC.

Unlike phosphofructokinase, adenylate cyclase (AC) activity increases after exposure to sphingosine.

Results:

We show that cAMP synthesis and ATP release are not failed in sphingosine-treated red blood cells in response to incubation with mastoparan 7, forskolin plus 3-isobutyl-1-methyl xanthine, agents that stimulate cAMP synthesis.

Conclusion:

Deoxy-hemoglobin, band 3, and AC are involved in the signaling pathway responsible for ATP released after sphingosine exposure.

Keywords: Band 3, Red blood cell, Sphingosine, ATP, cAMP, Hypoxia, Hemoglobin.