The Activity of α-glucosidase Inhibition of Pediococcus Acidilactici BAMA 4 Isolated from “Naniura” Traditional Foods from North Sumatera, Indonesia
Dewi Riastawaty1, Ermi Girsang2, Edy Fachrial2, Chrismis Novalinda Ginting2, Finna Piska3, *, Ali Napiah Nasution2, *
Identifiers and Pagination:Year: 2023
E-location ID: e1874091X2308100
Publisher ID: e1874091X2308100
Article History:Received Date: 14/02/2023
Revision Received Date: 05/07/2023
Acceptance Date: 17/07/2023
Electronic publication date: 25/09/2023
Collection year: 2023
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Type 2 diabetes is caused by unhealthy lifestyles, such as consuming foods rich in simple sugars and lack of exercise. One of the treatment therapies for this disease is α-glucosidase inhibitors. Some strains of the lactic acid bacteria (LAB) group typically exhibit α-glucosidase inhibitory activity, antimicrobial activity, acids and bile salt tolerance, and probiotic status.
This study aims to isolate LAB from naniura, characterize and test its activity as an α-glucosidase inhibitor, as well as identify those with the highest activity.
The method used to molecularly identify potentially good LAB was through the amplification of the 16S rRNA gene.
This study obtained a total of 9 strains using BAMA codes 1, 2, 3, 4, 5, 6, 7, 8, and 9. The cocci cell activity, gram-positive, and antibacterial activity of BAMA 4 against E.coli was 7.54 mm, while against S. aureus was 8.05 mm. The percentage of viability in acid and bile salt is 28.7% and 68.6%, respectively. Additionally, the proportion of α-glucosidase inhibition is 65%.
The BAMA 4 strain is a species of Pediococcus acidilactici. Pediococcus is a genus of gram-positive lactic acid bacteria in the Lactobacillacea family.
BAMA 4 strain produced antibacterial optimally and its cells survived the growth in acid and bile conditions. The percentage of probiotics was relevantly high in this activity. This study will be helpful for other in-vivo research.