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RESEARCH ARTICLE

Sphingosine Increases ATP Release From Red Blood Cells

Francesco Misiti1 , * Open Modal Authors Info & Affiliations
The Open Biochemistry Journal 31 May 2022 RESEARCH ARTICLE DOI: 10.2174/1874091X-v16-e2204210

Abstract

Background:

RBC plays a pivotal role in oxygen delivery, improving distribution where it needs. When RBC enters a low oxygen area, a mechanism mediated by a signaling pathway releases ATP, responsible for vasodilatation.

Objective:

Clarify the potential role of sphingosine on the release of ATP from RBC.

Methods:

ATP release increases after sphingosine exposure in RBC under low oxygen conditions. ATP release in deoxygenated RBC shows data like that of control RBC: (1) RBC after band 3 modification by 4,4'- diisothio-cyanato-stilbene- 2,2'-disulphonic acid (DIDS); (2) CO-treated RBC.

Unlike phosphofructokinase, adenylate cyclase (AC) activity increases after exposure to sphingosine.

Results:

We show that cAMP synthesis and ATP release are not failed in sphingosine-treated red blood cells in response to incubation with mastoparan 7, forskolin plus 3-isobutyl-1-methyl xanthine, agents that stimulate cAMP synthesis.

Conclusion:

Deoxy-hemoglobin, band 3, and AC are involved in the signaling pathway responsible for ATP released after sphingosine exposure.

Keywords: Band 3, Red blood cell, Sphingosine, ATP, cAMP, Hypoxia, Hemoglobin.
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