Purification, Characterization and Comparison between Two New L-asparaginases from Bacillus PG03 and Bacillus PG04



Mahsa Rahimzadeh*, 1, 4, Manijeh Poodat2, Sedigheh Javadpour3, Fatemeh Izadpanah Qeshmi4, Fereshteh Shamsipour5
1 Molecular Medicine Research Center, Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
2 Department of Biochemistry, Faculty of Sciences, Payame Noor University of Mashhad, Mashhad, Iran
3 Molecular Medicine Research Center, Department of Microbiology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
4 Food and Cosmetic Health Research Center, Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
5 Monoclonal Antibody Research Center, Avicenna Research Institute, (ACECR), Tehran, Iran


Article Metrics

CrossRef Citations:
1
Total Statistics:

Full-Text HTML Views: 485
Abstract HTML Views: 283
PDF Downloads: 134
ePub Downloads: 47
Total Views/Downloads: 949
Unique Statistics:

Full-Text HTML Views: 321
Abstract HTML Views: 184
PDF Downloads: 96
ePub Downloads: 38
Total Views/Downloads: 639



© Rahimzadeh et al.; Licensee Bentham Open

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Emam Hossein Boulevard, P.O. Box: 7919693116, Bandar Abbas, Iran; Tel: +98-761-6688024; Fax: +98-761-6668478; E-mail: mahsa_rahimzadeh@yahoo.com


Abstract

Background:

L-asparaginase has been used as a chemotherapeutic agent in treatment of lymphoblastic leukemia. In the present investigation, Bacillus sp. PG03 and Bacillus sp. PG04 were studied.

Methods:

L- asparaginases were produced using different culture media and were purified using ion exchange chromatography.

Results:

Maximum productivity was obtained when asparagine was used as the nitrogen source at pH 7 and 48 h after cultivation. New intracellular L-asparaginases showed an apparent molecular weight of 25 kDa and 30 kDa by SDS-PAGE respectively. These enzymes were active in a wide pH range (3-9) with maximum activity at pH 6 for Bacillus PG03 and pH 7 for Bacillus PG04 L-asparaginase. Bacillus PG03 enzyme was optimally active at 37 ˚C and Bacillus PG04 maximum activity was observed at 40˚C. Kinetic parameters km and Vmax of both enzymes were studied using L-asparagine as the substrate. Thermal inactivation studies of Bacillus PG03 and Bacillus PG04 L-asparaginase exhibited t1/2 of 69.3 min and 34.6 min in 37 ˚C respectively. Also T50 and ∆G of inactivation were measured for both enzymes.

Conclusion:

The results revealed that both enzymes had appropriate characteristics and thus could be a potential candidate for medical applications.

Keywords: Bacillus PG03, Bacillus PG04, L-asparaginase, Purification, Thermostability.