RESEARCH ARTICLE

Histidine Residues at the Active Site of the Pasteurella multocida Toxin

Gillian D Pullinger# Alistair J Lax, * Open Modal Authors Info & Affiliations
The Open Biochemistry Journal • 15 June 2007 • RESEARCH ARTICLE • DOI: 10.2174/1874091X00701010007

Abstract

We have investigated histidine residues near the active site of the mitogenic Pasteurella multocida toxin. Mutation of H1202 or H1228 had little effect, while the effect of mutation on H1223 depended on the amino acid substituted. Mutation of H1205 caused complete loss of activity, indicating its importance in PMT activity.

Keywords: Pasteurella multocida, toxin, PMT, domain structure, histidine residues, G-proteins.
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