Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust

Abdollah Gholami1, Golbabaei Farideh2, *, Gholamheidar Teimori3, Mojtaba Kianmehr4, Mehdi Yaseri5
1 Department of Occupational Health Engineering, School of Public Health, Birjand University of Medical Sciences, Iran
2 Department of Occupational Health, School of Public Health, Tehran University of Medical Sciences, Iran
3 Department of Environmental and Occupational Health Engineering, School of Public Health, Torbat Heydariyeh University of Medical Sciences, Iran
4 Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Iran
5 Epidemiology and Biostatistics, Tehran university of medical sciences, Iran


Background and Objectives:

Occupational exposure to silica dust can lead to biochemical damage. Malondialdehyde (MDA) can be considered as a primary marker for measuring the level of oxidative stress in a living organism. This study was conducted in order to evaluate the level of MDA in blood and urine of mice exposed to silica dust.

Material and Methods:

In this experimental study, 72 mice (BALB/c) were randomly allocated to five exposed groups and 1 control group. Exposure of mice to pure 99% silica dust was done in closed containers. Blood sampling was performed from the heart of mice and urine sampling fulfilled by insertion into a metabolic cage. The ROA et al. method was used to measure MDA.


The highest level of plasma MDA in group 1 in the 4th month was 8.4±0.41 nmol/l and the lowest level of MDA was 1.3±0.2 nmol/l in the third sampling in the control group, also the highest amount of urine MDA in the first and second groups and 4 months after exposure was 1.16±0.51 nmol/l, and the lowest in the control group and in the third sampling was 0.48±0.06 nmol/l. A significant difference was found between the levels of MDA in all exposed groups at different times except for the 5th group with the lowest concentration (P<0.05).


MDA in blood and urine could be proposed as a good biomarker for the evaluation of biochemical damages caused by silica dust. Measuring MDA is also a simple and inexpensive method that does not require complex equipment and can be used as an early detection test for biochemical damages caused by silica.

Keywords: Biomarker, Crystalline Silica, Dust, Mouse, Malondialdehyde.

Abstract Information

Identifiers and Pagination:

Year: 2019
Volume: 13
Publisher Item Identifier: EA-TOBIOCJ-2018-20

Article History:

Received Date: 05/12/2018
Revision Received Date: 05/03/2019
Acceptance Date: 10/03/2019
Electronic publication date: 20/03/2019
Collection year: 2019

© 2019 Gholami et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Correspondence: Address correspondence to this author at the Department of Occupational Health,School of Public Health, Tehran University of Medical Sciences, Iran; E-mail: